Identification and evaluation of potential microRNA markers for diagnostics in neurodegenerative diseases and correlation with other biochemical markers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F25%3AE0111934" target="_blank" >RIV/00843989:_____/25:E0111934 - isvavai.cz</a>

Alternative codes found

RIV/61988987:17110/25:A2603C2U

Result on the web

<a href="https://doi.org/10.1371/journal.pone.0333801" target="_blank" >https://doi.org/10.1371/journal.pone.0333801</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0333801" target="_blank" >10.1371/journal.pone.0333801</a>

Result language

angličtina

Original language name

Identification and evaluation of potential microRNA markers for diagnostics in neurodegenerative diseases and correlation with other biochemical markers

Original language description

Objectives: MicroRNAs (miRNA) are short, non-coding RNA molecules that play a crucial role in the development of organisms and are involved in various biological processes. They are considered potential biomarkers for many diseases, including neurodegenerative diseases. This study aimed to identify a set of microRNA targets that exhibited the greatest potential in successfully distinguishing and differentiating neurodegenerative diseases and to establish a correlation between selected miRNAs across different diagnostic groups. Methods: The study included the analysis of 126 patients. The patients were divided into five diagnostic groups - Alzheimer's disease, non-Alzheimer's dementia, Movement disorder, Dementia and movement disorder, and Healthy controls. The circulating RNA was isolated using the iCatcher Circulating cfRNA 1000 Kit with the iCatcher 12 automated isolator. The determination of microRNA was performed by TT-qPCR in the CFX96TM Real-Time Detection System. The concentrations of the remaining biomarkers were determined by ELISA. The statistical data were processed using MS Excel and MedCalc® software. Results: The following miRNAs were studied based on the primary screen for identification of potential microRNA targets and published literature data:hsa-miR-23a-3p, hsa-miR-29c-3p, hsa-miR-30b-5p, hsa-miR-142a-5p, hsa-miR-146a-5p, hsa-miR-151a-3p.A statistically significant correlation was identified between hsa-miR-29c-3p and hsa-miR-30b-5p, hsa-miR-30b-5p and hsa-miR-151a-3p, hsa-miR-23a-3p and hsa-miR-29c-3p, hsa-miR-23a-3p and hsa-miR-151a-3p, between hsa-miR23a-3p and hsa-miR-30b-5p, between hsa-miR-142a-5p and hsa-miR-146a-5p, hsa-miR-142a-5p and hsa-miR-151a-3p as well as between hsa-miR-146a-5p and hsa-miR-151a-3p.Significant differences were observed in hsa-miR-23a-3p and hsa-miR-29c-3p among different diagnostic groups. Compared to classical biomarkers of dementia, significant correlations were observed between plasmatic amyloid-ß peptide 42 ...

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10609 - Biochemical research methods

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

1932-6203

Volume of the periodical

20

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

16

Pages from-to

1-16

UT code for WoS article

001591829800044

EID of the result in the Scopus database

2-s2.0-105018397985