On‑the‑fy resolution enhancement in X‑ray protein crystallography using electric feld
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F10974938%3A_____%2F25%3A25_88_35" target="_blank" >RIV/10974938:_____/25:25_88_35 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s00249-025-01731-5" target="_blank" >https://doi.org/10.1007/s00249-025-01731-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00249-025-01731-5" target="_blank" >10.1007/s00249-025-01731-5</a>
Alternative languages
Result language
angličtina
Original language name
On‑the‑fy resolution enhancement in X‑ray protein crystallography using electric feld
Original language description
X-ray crystallography has tremendously served structural biology by routinely providing high-resolution 3D structures of macromolecules. The extent of information encoded in the X-ray crystallography is proportional to which resolution the crystals diffract and the structure can be refined to. Therefore, there is a continuous effort to obtain high-quality crystals, especially for those proteins, which are considered difficult to crystallize into high-quality protein crystals of suitable sizes for X-ray crystallography. Efforts in enhancing the resolution in X-ray crystallography have also been made by optimizing crystallization protocols using external stimuli such as an electric field and magnetic field during the crystallization. Here, we present the feasibility of on-the-fly post-crystallization resolution enhancement of the protein crystal diffraction by applying a high-voltage electric field. The electric field between 2 and 11 kV/cm, which was applied after mounting the crystals in the beamline, resulted in the enhancement of the resolution. The crystal diffraction quality improved progressively with the exposure time. Moreover, we also find that upto defined electric field threshold, the protein structure remains largely unperturbed, a conclusion further supported by molecular dynamics simulations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/EF16_019%2F0000789" target="_blank" >EF16_019/0000789: Advanced research using high intensity laser produced photons and particles</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Biophysics Journal
ISSN
0175-7571
e-ISSN
1432-1017
Volume of the periodical
54
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
89–95
UT code for WoS article
001401954300001
EID of the result in the Scopus database
2-s2.0-85217236850