The introduction to gene network influencing lupulin and some approaches to molecular study of hop metabolome regulation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F17%3AN0000068" target="_blank" >RIV/14864347:_____/17:N0000068 - isvavai.cz</a>

Result on the web

<a href="http://www.lfl.bayern.de/ipz/hopfen/024299/" target="_blank" >http://www.lfl.bayern.de/ipz/hopfen/024299/</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The introduction to gene network influencing lupulin and some approaches to molecular study of hop metabolome regulation

Original language description

MATOUŠEK, Jaroslav, Ajay Kumar MISHRA, Tomáš KOCÁBEK, Josef PATZAK, Mudra KHARE, G. S. DURAISAMY, Petr SVOBODA and Karel KROFTA. The introduction to gene network influencing lupulin and some approaches to molecular study of hop metabolome regulation. In: F. WEIHRAUCH, ed. Proceedings of the Scientific-Technical Commission 25 – 29 June 2017, St. Stefan am Walde, Austria. Wolnzach: Scientific-Technical Commission of the International Hop Growers´ Convention, 2017, pp. 82–83. ISSN 2512-3785. In our previous work we characterized several transcription factors (TFs) and TFs complexes that activate or suppress hop (Humulus lupulus L.) lupulin-specific genes involved in the regulation of prenylflavonoids. Among them there are regulatory genes from Myb, bHLH, WRKY, WD40 and bZIP families. The purpose of our work during several past years was to analyze some network connections especially within regulation of the final steps of prenylflavonoid biosynthesis involving genes encoding enzymes like chalcone synthase CHS_H1, prenyltransferase (Prt) and O-methyltransferase 1 (omt1). Several specific TF genes like HlMyb3 (e.g., MATOUŠEK et al. 2007; GATICA-ARIAS et al. 2013), HlMyb7 (MATOUŠEK et al. 2012), HlMyb8 (unpub.) and TF complexes like HlMyb3/HlbHLH2/HlWDR1, HlMyb2/HlbHLH2/HlWDR1 (MATOUŠEK et al. 2012) and HlWRKY1/HlWDR1 (WW) (MATOUŠEK et al. 2016) are of special interest and they were mapped in the initial regulátory network. In the case of WW complexes we described wide dependency of HlWRKY1 on phosphorylation, autoactivation and RNA silencing (MATOUŠEK et al. 2016). According to our recent analyses hop gene modulation by tripartite complexes is, in addition, strongly dependent on experimental light conditions enriched either by blue, red or UV-A spectra. Thus, all factors analysed so far suggest high complexity of lupulin regulatory network.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/GA13-03037S" target="_blank" >GA13-03037S: Combinatorial regulation and network of transcription factors involved in the biosynthesis of medicinal prenylflavonoids in hop (Humulus lupulus L.).</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů