Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00375145" target="_blank" >RIV/60077344:_____/11:00375145 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

Original language description

Complex lupulin gland-specific cDNA library from Osvald?s clone 72 hop was constructed to dissect regulation of biosynthetic pathway(s) leading to the accumulation of hop bitter acids and prenylflavonoids with promising health-beneficial activities. Fromthis cDNA library we isolated first hop-specific allelic isoforms of transcription factors (TF) HlbHLH2 and HlWDR1 which are involved in combinatorial control of lightresponsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWDR1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes with another novel hop TF HlMyb2 and previously isolated lupulin glandspecific HlMyb3. The complex formation leads to strong activation of chalconsynthase gene (chs_H1) promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accumulation of valuable metabolites of lupulin.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Brewing Science

ISSN

1613-2041

e-ISSN

—

Volume of the periodical

64

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

151-155

UT code for WoS article

—

EID of the result in the Scopus database

—