Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00340099" target="_blank" >RIV/60077344:_____/10:00340099 - isvavai.cz</a>
Alternative codes found
RIV/14864347:_____/10:#0000303 RIV/60076658:12310/10:00011808
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)
Original language description
TFs HlbZIP1A and HlbZIP2 cloned from a cDNA library based on glandular tissue-enriched hop cones are similar to the group A3 bZIP TFs according to characteristic domains. While HlbZIP1A is rather neutral (pl 6.42), HlbZIP2 is strongly basic (pl 8,51). Both bZIP TFs show specific expression in lupulin glands, although low expression was observed also in roots and immature pollen. In a transient expression system, both hop bZIP TFs activated the GUS-coupled promoters of chalcone synthase chs_H1 and O-methyl transferase 1, while the response of the valerophenone synthase promoter to the cloned hop TFs was very low, showing that the cloned bZIP TFs are not strictly G-box-specific. HPLC analysis of secondary metabolites in infiltrated Petunia hybrida showedthat both bZIPs interfere with the accumulation and the composition of flavonol glycosides, phenolic acids, and anthocyanins, suggesting the possibility of coregulating flavonoid biosynthetic pathways in hop glandular tissue.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Agricultural and Food Chemistry
ISSN
0021-8561
e-ISSN
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Volume of the periodical
58
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
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UT code for WoS article
000273671900032
EID of the result in the Scopus database
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