Cloning and Molecular Analyses of Hop Transcription Factors

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00336689" target="_blank" >RIV/60077344:_____/09:00336689 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cloning and Molecular Analyses of Hop Transcription Factors
Original language description
Expression of hop chs_H1 and other hop chalcon synthase (CHS)-like enzymes was assayed in developing pollen tissue. Both chs_H1 and valerophenone synthase (vps) are highly expressed in pollen suggesting the existence of common regulatory element(s). While vps is specific for female and male floral tissues, the oligofamily of chs_H1 is expressed in both generative and somatic tissues, suggesting a more universal promoter. Novel transcription factors (TFs) from bZIP and bHLH families involved in chs_H1 regulation have been found by means of genomic and cDNA libraries screening. These include HlbZIP1A and its truncated variant HlbZIP1B, HlbZIP2 and HlbHLH. The activation potential of HlbZIP2 and previously cloned hop TFs were analysed in P.hybrida and N.benthamiana transient expression systems. HlbZIP2, as well as both HlMyb1 and HlMyb3 were found as activators of chs_H1 promoter. In combinatorial system, HlbZIP2 was found to modulate the expression of both Myb TFs from hop.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the Second International Humulus Symposium
ISBN
978-90-6605-722-7
ISSN
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e-ISSN
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Number of pages
8
Pages from-to
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Publisher name
ISHA Acta Horticulturae
Place of publication
Ghent
Event location
Ghent
Event date
Sep 1, 2009
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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