Cloning and molecular analyses of hop transcription regulation factors.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F09%3A%230000230" target="_blank" >RIV/14864347:_____/09:#0000230 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cloning and molecular analyses of hop transcription regulation factors.
Original language description
To identify novel transcription factors involved in the regulation of chs_H1 genes in lupulin glands, cone-specific ESTs showing homology to bZIP and bHLH were utilized to screen hop cDNA and genomic libraries. Additionally, inverse PCR was used to amplify full length cDNAs of members of two bZIP oligofamilies from hop. Transcription factors HlbZIP1A and HlbZIP2 have predicted molecular masses of 27.4 and 34.2 kDa, respectively. While HlbZIP1A is rather neutral (pI 6.42), HlbZIP2 is strongly basic (pI 8.51). A truncated variant of HlbZIP1 (HlbZIP1B) that is strongly basic but lacks the zipper domain was also cloned from hop. Additionally, the first bHLH transcription factor was cloned from hop. HlbHLH1 is a protein of 33.6 kDa with a predicted pI of 6.08. Based on semiquantitative RT PCR results, this TF is expressed specifically in hop floral and cone tissue.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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