Preliminary Results of In Vitro Thermotherapy of Pear Cultivars

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F09%3A%230000177" target="_blank" >RIV/25271121:_____/09:#0000177 - isvavai.cz</a>
Result on the web
<a href="http://www.scopus.com/inward/record.url?eid=2-s2.0-75649149476&partnerID=40&md5=ac5996f11e4672ed151669acf899f597" target="_blank" >http://www.scopus.com/inward/record.url?eid=2-s2.0-75649149476&partnerID=40&md5=ac5996f11e4672ed151669acf899f597</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Preliminary Results of In Vitro Thermotherapy of Pear Cultivars
Original language description
Two pear cultivars Alexander Lucas and Elektra were selected for application of in vitro thermotherapy at 39 °C as a technique for virus elimination. Trees were selected and the presence of viruses was initially determined before thermotherapy by ELISA and RT-PCR . Based on the results of these tests, both pear cultivars were infected with Apple stem pitting virus (ASPV). Cultivars were successfully multiplied using in vitro cultures on MS medium with 1.5 mg/L BAP. Heat treatment (39°C) was applied to the in vitro cultures on the same MS medium with 1.5 mg/L BAP as for multiplication. Between five and 10 days of heat therapy, the green apical parts of the axis, comprising the apical meristem plus one or two leaf primordia, were dissected and transferred to a fresh multiplication MS medium. For Elektra, 100% and for Alexander Lucas, 79.2% of isolated apices survived and developed shoots. Six months after the end of heat treatment, eight clones of pear cultivar Elektra and seven clones o
Czech name
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Czech description
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Project
<a href="/en/project/1B44051" target="_blank" >1B44051: Research and development of standard methods of recovering cultivars of fruit trees and grapevine from viruses, phytoplasmas and quarantine pathogens by thermotherapy and in vitro culture for certification system of healthy state of planted material.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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