Elimination of Apple Stem Pitting Virus from Pear Cultivars by in Vitro Chemotherapy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F11%3A%230000165" target="_blank" >RIV/25271121:_____/11:#0000165 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Elimination of Apple Stem Pitting Virus from Pear Cultivars by in Vitro Chemotherapy
Original language description
The objective of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Apple stem pitting virus (ASPV) from in vitro grown plants of infected pear cultivars Alexander Lucas, Bohemica, Elektra and Rote Williams. ASPV was proved in selected field-grown trees of these cultivars by repeated RT-PCR testing. In vitro cultures were successfully multiplied on MS medium with BAP (6-benzylaminopurine). Ribavirin was added in concentration 20 mg/L after autoclaving to thesame MS medium as for multiplication. After a subculture period of 4 weeks, the apical part of the axis about 3 mm in length comprising the apical meristem plus two to three leaf primordia, which developed during chemotherapy, was dissected under a laminar flow hood and transferred to a fresh multiplication MS medium with 1.5 mg/L BAP for regeneration. From the selected genotypes, low phytotoxicity of ribavirin in concentration 20 mg/L was observed only in the case of cultivars Alexander
Czech name
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Czech description
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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