In Vitro Chemotherapy of Pear Cultivars

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F14%3A%230000583" target="_blank" >RIV/25271121:_____/14:#0000583 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
In Vitro Chemotherapy of Pear Cultivars
Original language description
The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Apple stem pitting virus (ASPV) from in vitro plants of infected pear cultivars 'Astra', 'David' and 'Erika'. According to RT-PCR testing, selectedfield-grown trees of all pear cultivars were infected with ASPV. In vitro cultures were successfully multiplied on MS medium with BAP (6-benzylaminopurine). Ribavirin was used at 20 mg L-1 for virus elimination. Low phytotoxicity of ribavirin at 20 mg L-1 was observep only for the cultivar 'David'. Six of the twenty shoot tips of 'David' showed apex necrosis and died after transfer to regeneration medium. The success of chemotherapy after RT-PCR testing of ribavirin treated plants varied between 65.0%for cultivars 'Astra' and 'Erika' and 71.4% for cultivar 'David'. The results achieved and the relatively high percentage of virus free plants obtained after 6 months of in vitro cultivation confirm the effectiveness of ribavirin in elimi
Czech name
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Czech description
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Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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