Results of in vitro chemotherapy of apple cv. Fragrance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F13%3A%230000474" target="_blank" >RIV/25271121:_____/13:#0000474 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Results of in vitro chemotherapy of apple cv. Fragrance

Original language description

The effect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. The results of ELISA and RT-PCR testing proved the presence of mixed infection ofApple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial field-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with well-developed leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infectedwith ACLSV only after the first chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. The success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after th

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Horticultural Science

ISSN

0862-867X

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

186-190

UT code for WoS article

000329605000007

EID of the result in the Scopus database

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