Elimination of PDV from sweet cherry cultivars by in vitro chemotherapy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000098" target="_blank" >RIV/25271121:_____/19:N0000098 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1242.4" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1242.4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1242.4" target="_blank" >10.17660/ActaHortic.2019.1242.4</a>

Result language

angličtina

Original language name

Elimination of PDV from sweet cherry cultivars by in vitro chemotherapy

Original language description

The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Prune dwarf virus (PDV) from in vitro grown plants of infected sweet cherry cultivars ‘Tamara’ and ‘Amid’. Initial in vitro cultures were grown in Erlenmeyer flasks with 25 mL of MS medium (Murashige and Skoog, 1962), to which 1.5 mg L-1 BAP (6-benzylaminopurine) had been added. All cultures were cultivated in controlled environment chambers at 22±1°C. As soon as enough shoots were developed, individual shoots (5-10 mm in length) were excised from stock collections and transferred to treatment media with ribavirin. Ribavirin was added in concentration of 20 mg L-1 after autoclaving to the same MS medium as for multiplication. After a subculture period of 4 weeks, the apical part of the axis (about 3 mm in length comprising the apical meristem plus two-three primordial leaves) was dissected under a laminar flow hood and transferred to a fresh multiplication MS medium with 1.5 mg L-1 BAP for regeneration. The chemotherapy technique was able to eliminate PDV in high percentage according to the cultivar, ranging between 100.0 and 96.4% for cultivars ‘Tamara’ and ‘Amid’, respectively. In the course of chemotherapy cycles, ribavirin concentrations of 20 mg L-1, ‘Amid’ plants did not display symptoms of phytotoxicity and appeared vigorous and healthy. In contrary, 35% of ‘Tamara’ in vitro plants did not survive the ribavirin treatment. The obtained results demonstrate the effectiveness of the method to eliminate PDV by a combination of in vitro cultures, chemotherapy with ribavirin and subsequent removing of apical meristematic region.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Acta Horticulturae

ISBN

978-94-62612-39-6

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

31-34

Publisher name

ISHS

Place of publication

Leuven

Event location

Chania, Kréta, Řecko

Event date

Jul 10, 2019

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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