Improvement of System for Testing of Pome Fruit resistance to Erwinia amylovora Using In Vitro Artificial Inoculation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F14%3A%230000585" target="_blank" >RIV/25271121:_____/14:#0000585 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Improvement of System for Testing of Pome Fruit resistance to Erwinia amylovora Using In Vitro Artificial Inoculation
Original language description
Fire blight is a destructive bacterial disease that presents potential thr eat to apple and pear industries throughout Europe. Specific, safe and reliable methods for identifying the pathogen Erwinia amylovora and a consistent system for cultivar resistance testing can be very important factors in the management of the disease. Therefore a screening system for testing of pome fruit resistance to E. amylovora was developed based on in vitro artificial inoculation. Selected pome fru it genotypes were successfully established in vitro using mercuric chloride in a concentration of O.I5% as a sterilization solution. MS medium according to Murashige and Skoog (I962) with addition of cytokinins 6-benzylaminopurine (BAP, 2 and 4 mg/L) or thidiazuron (TDZ, 0.5and I mg/L) proved to be suitable for in vitro proliferation. We obtained multiplication coefficients higher than 2.0 for all tested pome fruit genotypes. The application of TDZ in both concentrations induced hyperhydricity in the case of
Czech name
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Czech description
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Project
<a href="/en/project/QJ1210184" target="_blank" >QJ1210184: The utilization of biotechnological methods for more effective testing of pome fruit resistance to fire blight (bacterium Erwinia amylovora)</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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