Micropropagation of Czech Apple Cultivars

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F15%3A%230000605" target="_blank" >RIV/25271121:_____/15:#0000605 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Micropropagation of Czech Apple Cultivars
Original language description
The objective of this study was to investigate micropropagation protocols for use with three apple cultivars (James Grieve Compact, Jarka and Mivibe) bred in the Czech Republic. Apple shoot tips (5 to 10 mm in length) were established in vitro using 0.15% HgCl2 as sterilization solution. Contamination rate, survival and development of shoots from excised shoot tips were analyzed after sterilization. For the three cultivars, the effect of three growth regulators 1, 2 and 4 mg L-1 BAP (6- benzylaminopurine), 0.5 and 1 mg L-1 TDZ or 10 mg L-1 2iP in basal MS medium on proliferation, callus formation and shoot morphology is shown. Values of proliferation rate varied between 1.1 and 3.6. Generally, the highest rate was obtained for cultivar Jarka that produced 3.6 new shoots on MS medium containing 4 mg L-1 BAP. The lowest number of newly formed shoots (1.1) was noted for cultivars James Grieve Compact and Mivibe on MS medium with low concentration of BAP (1 mg L-1). In vitro plants of Jame
Czech name
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Czech description
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Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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