In vitro establishment and proliferation of apple cultivars

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F16%3AN0000010" target="_blank" >RIV/25271121:_____/16:N0000010 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1113.15" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2016.1113.15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1113.15" target="_blank" >10.17660/ActaHortic.2016.1113.15</a>

Result language

angličtina

Original language name

In vitro establishment and proliferation of apple cultivars

Original language description

The goal of this study was to investigate in vitro multiplication protocols for the use with apple cultivars 'Bohemia, 'Rubimeg' and 'Primadela' grown in the Czech Republic. For the initiation phase, dormant shoots (15-20 cm in length) were collected from field germplasm at Research and Breeding of Pomology Holovousy Ltd. Shoot tips (5 to 10 mm in length) were successfully established in vitro using mercuric chloride at a concentration of 0.15% as a sterilization solution. To assess the effect of plant growth regulators on multiplication, in vitro shoots were cultured on the basal MS medium supplemented with 1, 2 and 4 mg L-1 6-benzylaminopurine (BAP), 0.5 and 1 mg L-1 thidiazuron (TDZ), or 1, 2 and 4 mg L-1 kinetin. BAP at the highest concentrations (2 and 4 mg L-1) and TDZ were found to be more effective than kinetin for shoot multiplication. Generally, the highest proliferation rate was obtained for 'Bohemia' that produced 4.1 shoots (longer than 10 mm) on medium containing 4 mg L-1 BAP. On the contrary, the lowest proliferation rate (1.0) was obtained for 'Rubimeg' and 'Primadela', which almost did not multiply on MS medium containing the lowest concentration of BAP (1 mg L-1). Kinetin at the tested concentrations did not promote satisfactorily proliferation of the three apple cultivars (proliferation between 1.1 and 1.3). Efficient micropropagation protocols are a prerequisite for utilization of sophisticated in vitro virus elimination techniques (in vitro thermotherapy and chemotherapy).

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

GC - Plant growing, crop rotation

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the International Symposium on Micropropagation and In Vitro Techniques

ISBN

978-94-62611-07-8

ISSN

0567-7572

e-ISSN

—

Number of pages

5

Pages from-to

107-111

Publisher name

International Society for Horticultural Science

Place of publication

Leuven, Belgium

Event location

Brisbane, Australia

Event date

Jan 1, 2014

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000391238600015