ldentification of a cytokinin treatment for in vitro propagation of two sweet cherry cultivars in preparation of a sanitation protocol

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F17%3AN0000009" target="_blank" >RIV/25271121:_____/17:N0000009 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1155.22" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2017.1155.22</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1155.22" target="_blank" >10.17660/ActaHortic.2017.1155.22</a>

Result language

angličtina

Original language name

ldentification of a cytokinin treatment for in vitro propagation of two sweet cherry cultivars in preparation of a sanitation protocol

Original language description

Sweet cherry (Prunus avium L.), member of the subgenus Cerasus, is a globally important vegetatively propagated fruit crop. Unfortunately, trees grown in fields and nurseries are threatened by the Prune dwarf virus (PDV). This vir us causes malformations and a decrease in growth vigour and yield. A solution to get rid of the PDV virus in the Czech prospective sweet cherry cultivars 'Tamara' and 'Kasandra' is a part of the global sanitation programme. Tissue culture techniques are an important tool for quick multiplication of requested cultivars and offer the opportunity to improve the health status of micropropagated plants by combining in vitro culture with chemo or thermotherapy. The goal of this study was to identity factors affecting the ability of Czech sweet cherry cultivars to produce shoots under in vitro culture conditions. Six proliferation MS media containing 1, 2 and 4 mg L·l BAP (6-benzylaminopurine), O.S and 1 mg L·l TDZ (thidiazuron) or 10 mg L·t ZiP (6-(g,gdimethylallylamino) purine) were tested. Values of multiplication r ate varied between 1.2 and 4.5. From the tested cytokinins, BAP at a concentration of 4 mg L·t was found to be more effective than TDZ and ZiP for shoot multiplication. In conclusion, our experiments confirmed that in vitro propagation of selected sweet cherry cultivars can be achieved. The described procedure enabled us to multiply and maintain in vitro plants throughout the year of the two sweet cultivars for further experiments with in vitro chemotherapy.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QJ1210175" target="_blank" >QJ1210175: Research and development of standard methodic procedures of recovering of fruit trees and grapevinesby chemotherapy of in vitro cultures for certification system of healthy status of planting material.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Acta Horticulturae

ISBN

978-94-6261-151-1

ISSN

0567-7572

e-ISSN

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Number of pages

4

Pages from-to

161-164

Publisher name

ISHS

Place of publication

Leuven 1, Belgium

Event location

Sanremo, Italy

Event date

Apr 1, 2015

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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