Evaluation of genetic diversity within sweet cherry accessions of Czech genetic resources by molecular SSR markers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000095" target="_blank" >RIV/25271121:_____/19:N0000095 - isvavai.cz</a>
Alternative codes found
RIV/14864347:_____/19:N0000014
Result on the web
<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.9" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1235.9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.9" target="_blank" >10.17660/ActaHortic.2019.1235.9</a>

Result language
angličtina
Original language name
Evaluation of genetic diversity within sweet cherry accessions of Czech genetic resources by molecular SSR markers
Original language description
Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of individual accessions, so that we eliminated duplications and characterized genetic relationships. Recently, there are many different molecular methods but the most reliable are SSR markers.In our work, we used PCR primer combinations for 20 SSR and 2 EST-SSR markers for analyses of 82 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 113 polymorphic markers were amplified which we used for hierarchical cluster analysis of genetic variability. The result dendrogram corresponded with genealogical and geobotanical characteristics of individual accessions which were divided into five clusters. There was also proved the breeding history of several known accessions. This work was supported by the National Agency for Agricultural Research of the Ministry of Agriculture of CR in project QJ1510001.
Czech name
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Czech description
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Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Project
<a href="/en/project/QJ1510001" target="_blank" >QJ1510001: Research of sweet cherry germplasm by molecular-genetic methods</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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