Determination of self-incompatible genotypes in sweet cherry accessions of Czech genetic resources.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000096" target="_blank" >RIV/25271121:_____/19:N0000096 - isvavai.cz</a>

Alternative codes found

RIV/14864347:_____/19:N0000017

Result on the web

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.52" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1235.52</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.52" target="_blank" >10.17660/ActaHortic.2019.1235.52</a>

Result language

angličtina

Original language name

Determination of self-incompatible genotypes in sweet cherry accessions of Czech genetic resources.

Original language description

Sweet cherries are self-incompatible determined by a gametophytic selfincompatibility system (GSI). The self-incompatibility is controlled by a multi-allelic Slocus and includes two genes coding for the synthesis of proteins responsible for the incompatibility response. The knowledge about the S-allele constitution of cultivars is very important for fruit growers and breeders. Recently, molecular methods have been developed to distinguish the S-alleles in sweet cherries and different molecular primers for the PCR-based identification of all S-alleles were designed during the last years. In our work, we analyzed S-locus genotypes by 10 universal and specific PCR primer combinations within 82 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. We confirmed previous S-genotyping for 46 accessions excepted Droganova, Hedelfingen, Erika Frühe Meckenheimer, Huldra, Rivan, Vanda and Winkler. It can be due to mislabelling or mistakes in previous analyses. Newly, S-genotyping was determined for 36 accessions when we found 4 new S-loci combinations. Totally, we detected S-locus combinations in 21 incompatibility group. The most frequent incompatibility groups were III (S3S4), IV (S2S3), VI (S3S6) and II (S1S3) with 13, 11, 9 and 8 genotypes, respectively.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Project

<a href="/en/project/QJ1510001" target="_blank" >QJ1510001: Research of sweet cherry germplasm by molecular-genetic methods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Acta Horticulturae

ISBN

9789462612327

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

379–385

Publisher name

ISHS

Place of publication

Leuven

Event location

Yamagata, Japonsko

Event date

Jun 5, 2017

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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