Determination of sweet and sour cherry (in)compatibility in one PCR and fragment analysis reaction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F23%3AN0000066" target="_blank" >RIV/25271121:_____/23:N0000066 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of sweet and sour cherry (in)compatibility in one PCR and fragment analysis reaction

Original language description

Presentation of a poster at the XVI EUCARPIA Symposium on Fruit Breeding and Genetics, Dresden-Pillnitz (Germany), 11.9.2023 - 16.9.2023. Both sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.) possess so-called S-alleles determining their self-(in)compatibility. In case of self-incompatibility, suitably chosen pollinizers with distinct S-alleles have to be planted in orchards to ensure a high fruit set, however, harvest may be hampered by biotic and abiotic stresses that may influence successful pollination. Management of an orchard with two cultivars planted may be also complicated. Knowledge of S-alleles is therefore essential in all cultivated cherries, and self-compatible genotypes are obviously preferred. However, S-alleles determination is a challenging process run usually in several PCR and fragment analysis reactions as up-to-date 54 S-alleles have been identified in sweet cherries and 30 S-alleles in sour cherries. A novel kit has been developed to identify all known sweet cherry S-alleles including self-compatible ones in only one multiplex PCR and fragment analysis, and a similar kit for sour cherry is currently being optimized. Both assays are specific and reliable, enabling to distinguish also S-alleles differing by only one nucleotide mismatch (i.e. S5 vs S5´ in sweet cherry, S13 and S13´ in sour cherry). One-reaction analysis format is especially advantageous since it saves labor, time and cost of the analysis. The kits can be used for routine S-allele determination especially in unknown genotypes or free pollination selections or for marker-assisted selection of self-compatible genotypes.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Project

<a href="/en/project/TN01000062" target="_blank" >TN01000062: Biotechnological centre for plant genotyping</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů