High throughput sequencing testing of certified apple M9 rootstocks
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F23%3AN0000089" target="_blank" >RIV/25271121:_____/23:N0000089 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17660/ActaHortic.2023.1378.51" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2023.1378.51</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2023.1378.51" target="_blank" >10.17660/ActaHortic.2023.1378.51</a>
Alternative languages
Result language
angličtina
Original language name
High throughput sequencing testing of certified apple M9 rootstocks
Original language description
A project on high throughput sequencing (HTS) screening of certified rootstocks for virus-like agents (virus, viroid, phytoplasma) started in 2019. The aim is to acquire apple propagation material free of the virus-like agents with qualitatively new knowledge about its health status using HTS. Twenty-eight M9 rootstock plants labelled as standard CZ, elite, and basis material were purchased from a Czech and two international producers and were HTS tested. The HTS libraries were prepared from enriched double-stranded RNA extracts and processed with NovaSeq 6000. All HTS tested plants were free of Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV), Candidatus Phytoplasma mali (Apple proliferation phytoplasma), and Apple scar skin viroid (ASSVd) and complied with the EPPO certification standard (Anonymous, 1999). The results were verified using conventional RT-PCR. All elite rootstocks were free of the virus-like agents. In the standard CZ and basis rootstocks, we detected Apple hammerhead viroid-like RNA (AHVd), Citrus concave gum-associated virus (CCGaV) and several sequences of three tentative novel viruses, which are subjects of further studies. CCGaV and AHVd mixed infections were reported in apple in Hungary (Jahan et al., 2019) and CCGaV in USA (Wright et al., 2018). Our results suggest the necessity for more rigorous HTS testing of rootstock source plants to determine their health status.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/QK1910065" target="_blank" >QK1910065: New approaches to the production of certified varieties of apple trees using NGS diagnostics of pathogens</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Acta Horticulturae
ISBN
978 94 6261 376 8
ISSN
0567-7572
e-ISSN
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Number of pages
5
Pages from-to
391-395
Publisher name
ISHS
Place of publication
Leuven, Belgium
Event location
Angers, France
Event date
Aug 14, 2022
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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