Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F15%3A%230000832" target="_blank" >RIV/25328859:_____/15:#0000832 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.4238/2015.April.27.14" target="_blank" >http://dx.doi.org/10.4238/2015.April.27.14</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4238/2015.April.27.14" target="_blank" >10.4238/2015.April.27.14</a>

Result language

angličtina

Original language name

Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system

Original language description

HTS-1 is a new kind of pistillody wheat. All or parts of its stamen are transformed into pistils or pistil-like structures, and it has more seed sets per floret than normal wheat under normal cultivation conditions. To investigate the expression divergence in this mutant, an annealing control primer system was used to identify differentially expressed genes (DEGs) in the young spikelets. As a result, three DEGs, including HDB2, HGF2, and HCG4, were detected, with variable expression in HTS-1 and the control. After further confirmation using real-time reverse transcription polymerase chain reaction analysis, these genes were overexpressed in HTS-1 wheat. NGF2 was identified in the double ridge to floret differentiation stages; HDB2 and HCG4 were identified in the stage of pistil and stamen-differentiating. Therefore, we inferred that the homeotic transformation of stamens into pistil-like structures occurred during the early stage of stamen development. Sequence alignment analysis revealed that HDB2 encodes a putative protein of 189 amino acids, with high homology to the DEAD-box ATP-dependent RNA helicase, and HCG4 was identical to the Chinese spring wheat cDNA clone predicted protein according to GenBank. However, NGF2 was not found to have significant similarity to any reported proteins, suggesting it is a new functional gene in wheat. The results suggest that HDB2, HCG4, and HGF2 are minor genes contributing to pistillody trait formation in HTS-1.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/QJ1510206" target="_blank" >QJ1510206: Research of the wheat different grain discoloration types caused by substances with antioxidant effects, their use for development of varieties with positive health benefit for human and animal nutrition</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

GENETICS AND MOLECULAR RESEARCH

ISSN

1676-5680

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

2

Country of publishing house

BR - BRAZIL

Number of pages

10

Pages from-to

3995-4004

UT code for WoS article

000357183700115

EID of the result in the Scopus database

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