Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F25%3AN0000009" target="_blank" >RIV/25328859:_____/25:N0000009 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41477-025-02110-w" target="_blank" >https://www.nature.com/articles/s41477-025-02110-w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41477-025-02110-w" target="_blank" >10.1038/s41477-025-02110-w</a>
Alternative languages
Result language
angličtina
Original language name
Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping
Original language description
Protecting crops from diseases is vital for the sustainable agricultural systems that are needed for food security. Introducing functional resistance genes to enhance the plant immune system is highly effective for disease resistance, but identifying new immune receptors is resource intensive. We observed that functional immune receptors of the nucleotide-binding domain leucine-rich repeat (NLR) class show a signature of high expression in uninfected plants across both monocot and dicot species. Here, by exploiting this signature combined with high-throughput transformation, we generated a wheat transgenic array of 995 NLRs from diverse grass species to identify new resistance genes for wheat. Confirming this proof of concept, we identified new resistance genes against the stem rust pathogen Puccinia graminis f. sp. tritici and the leaf rust pathogen Puccinia triticina, both major threats to wheat production. This pipeline facilitates the rapid identification of candidate NLRs and provides in planta gene validation of resistance. The accelerated discovery of new NLRs from a large gene pool of diverse and non-domesticated plant species will enhance the development of disease-resistant crops.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Plants
ISSN
2055-026X
e-ISSN
2055-0278
Volume of the periodical
11
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
15
Pages from-to
2100-2114
UT code for WoS article
001576816900001
EID of the result in the Scopus database
2-s2.0-105016887175