The loss of polyphenol oxidase function is associated with hilum pigmentation and has been selected during pea domestication
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26296080%3A_____%2F22%3AN0000135" target="_blank" >RIV/26296080:_____/22:N0000135 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/22:73613916
Result on the web
<a href="https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.18256" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.18256</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/nph.18256" target="_blank" >10.1111/nph.18256</a>
Alternative languages
Result language
angličtina
Original language name
The loss of polyphenol oxidase function is associated with hilum pigmentation and has been selected during pea domestication
Original language description
Seed coats serve as protective tissue to the enclosed embryo. As well as mechanical there are also chemical defence functions. During domestication, the property of the seed coat was altered including the removal of the seed dormancy. We used a range of genetic, transcriptomic, proteomic and metabolomic approaches to determine the function of the pea seed polyphenol oxidase (PPO) gene. Sequencing analysis revealed one nucleotide insertion or deletion in the PPO gene, with the functional PPO allele found in all wild pea samples, while most cultivated peas have one of the three nonfunctional ppo alleles. PPO functionality cosegregates with hilum pigmentation. PPO gene and protein expression, as well as enzymatic activity, was downregulated in the seed coats of cultivated peas. The functionality of the PPO gene relates to the oxidation and polymerisation of gallocatechin in the seed coat. Additionally, imaging mass spectrometry supports the hypothesis that hilum pigmentation is conditioned by the presence of both phenolic precursors and sufficient PPO activity. Taken together these results indicate that the nonfunctional polyphenol oxidase gene has been selected during pea domestication, possibly due to better seed palatability or seed coat visual appearance.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/GA19-07155S" target="_blank" >GA19-07155S: Identification of regulatory networks controlling pea seed coat development using combination of RNA sequencing, protein and metabolites analysis.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Phytologist
ISSN
0028-646X
e-ISSN
1469-8137
Volume of the periodical
235
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
1807-1821
UT code for WoS article
000809206100001
EID of the result in the Scopus database
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