Rapid testing of clopidogrel resistance by genotyping of CYP2C19 and CYP2C9 polymorphisms using denaturing on-chip capillary electrophoresis.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26475821%3A_____%2F12%3A%230000107" target="_blank" >RIV/26475821:_____/12:#0000107 - isvavai.cz</a>

Alternative codes found

RIV/00064173:_____/12:43896090 RIV/00216208:11120/12:43896090

Result on the web

<a href="http://onlinelibrary.wiley.com.ezproxy.is.cuni.cz/doi/10.1002/elps.201100457/pdf" target="_blank" >http://onlinelibrary.wiley.com.ezproxy.is.cuni.cz/doi/10.1002/elps.201100457/pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Rapid testing of clopidogrel resistance by genotyping of CYP2C19 and CYP2C9 polymorphisms using denaturing on-chip capillary electrophoresis.

Original language description

Antiplatelet therapy is a cornerstone of cardiovascular treatment in patients with coronary artery disease and after myocardial infarction. Clopidogrel has become a popular antiplatelet agent due to its fast action and low frequency of adverse effects. Kinetics of clopidogrel metabolism is driven by enzymatic activity of the Cytochrome P450 system. Genotyping of CYP2C19 and CYP2C9 polymorphisms allows to identify slow metabolizers showing resistance to clopidogrel therapy. Here, we describe a new alternative genotyping approach combining the separation power of denaturing capillary electrophoresis with the analysis speed and ease of use of Bioanalyzer chipCE platform. Using an upgraded heater control, we present an optimization for allele separation ofCYP2C19 I331V, CYP2C9 R144C, and CYP2C9 I359L polymorphisms employing run temperatures of up to 55 degrees C.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FA - Cardiovascular diseases including cardio-surgery

OECD FORD branch

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Project

<a href="/en/project/NS9988" target="_blank" >NS9988: Resistance to antiplatelet agents, its etiology and the possibility of its affection</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ELECTROPHORESIS

ISSN

0173-0835

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

1306-1310

UT code for WoS article

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EID of the result in the Scopus database

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