Effect of selenized Streptococcus thermophilus, Enterococcus faecium and Saccharomyces cerevisiae on cytotoxicity and adherence to Caco-2/HT29 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F22%3AN0000085" target="_blank" >RIV/26722861:_____/22:N0000085 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of selenized Streptococcus thermophilus, Enterococcus faecium and Saccharomyces cerevisiae on cytotoxicity and adherence to Caco-2/HT29 cells

Original language description

Selenium, as one of the essential microelements for the human body, is important for the proper functioning of organisms, maintenance of the immune system and antioxidant activity. Some bacteria and yeasts are capable of accumulating inorganic selenium form (selenite, selenate) from the environment and transforming them into organic forms, such as seleno-amino acids. Organic forms are less toxic, more bioavailable and utilizable by the human body. Therefore we decided to evaluate the ability of inorganic Se to accumulate and biotransform in Streptococcus thermophillus CCDM 144, Enterococcus faecium CCDM 922A and Saccharomyces cerevisiae CCDM 272. Tested MOs were cultivated in medium enriched with sodium selenite at concentrations of 10 and 30 mg/L. Subsequently, cytotoxicity of selenized strains to human adenocarcinoma Caco-2 and HT29 cells and their ability to adhere was determined. Strains selenized with 30 mg/L of sodium selenite significantly reduced the viability of Caco2 and HT-29 cells by 40–75 % compared with the lower (10 mg/L). The effect of selenium enrichment on adherence is strain-specific. Higher concentration of accumulated selenium by Saccharomyces cerevisiae CCDM 272 led to increase of adherence ability. The opposite result was determined in case of Streptococcus thermophilus CCDM 144. The results indicate that 10 mg/L sodium selenite is a suitable concentration for balance between tested strains tolerance to Se and their potential cytotoxic effect on the cell tissue model. Citation: Mrvikova I., Hyrslova I., Krausova G., Kana A., Kantorova V., Doskocil I.: Effect of selenized Streptococcus thermophilus, Enterococcus faecium and Saccharomyces cerevisiae on cytotoxicity and adherence to Caco-2/HT29 cells. Ostatní – Poster a abstrakt ve sborníku. Conference: 11th Central European Congress on Food and Nutrition “Food, technology and nutrition for healthy people in a healthy environment“ CEFood 2022, Čatež ob Savi, Slovenia, 27th – 30th September 2022, Abstract Book CEFood Congress Book „Food, technology and nutrition for healthy people in a healthy environment“ pp. 230

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/LTC20014" target="_blank" >LTC20014: Research of selenium-enriched lactic acid bacteria, bifidobacteria and yeasts and influence of low-pH stress exposure on their selenium biotransformation efficiency.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů