Determination of selenium nanoparticles in fermented dairy products

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F23%3AN0000001" target="_blank" >RIV/26722861:_____/23:N0000001 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22340/23:43925924

Result on the web

<a href="https://doi.org/10.1016/j.sab.2022.106592" target="_blank" >https://doi.org/10.1016/j.sab.2022.106592</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sab.2022.106592" target="_blank" >10.1016/j.sab.2022.106592</a>

Result language

angličtina

Original language name

Determination of selenium nanoparticles in fermented dairy products

Original language description

A single particle inductively coupled plasma mass spectrometry (sp-ICP-MS) method with improved sensitivity for determination of selenium nanoparticles (SeNPs) in lactic acid bacteria, bifidobacteria, and yeast was developed. The carbon-induced signal enhancement using 1% (v/v) methanol for preparation of sample dispersion led to decreasing detection limits from 61 nm to 47 nm in comparison to pure aqueous dispersion. Accuracy of SeNPs’ size and number concentration determination were tested by adding artificially prepared SeNPs to the cells dispersion. While the found size of the SeNPs corresponded to original value, the number concentration recovery ranged from 46 to 102%. The repeatability of SeNPs size measurement was 0.6–15% and of number concentration determination 2.5–54%. The spherical and ovoid shaped SeNPs with average diameter in range of 60–261 nm and number concentration of 2.4 × 109–1.6 × 1011 mL-1 located both extracellularly and intracellularly was found by transmission electron microscopy and sp-ICP-MS in cell cultures growing in medium containing 10 mg L-1 of sodium selenite. Bio-synthesized SeNPs were stable for one week in aqueous dispersion and their stability was prolonged to one month by addition of hydrochloric acid (pH = 1.5). Moreover, the possibility of analyzing SeNPs in enzymatic extracts intended for speciation analysis of organic selenium species was proven to be suitable also for SeNPs characterization. Thus, a way to share sample preparation procedure with different types of analyses was demonstrated.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/LTC20014" target="_blank" >LTC20014: Research of selenium-enriched lactic acid bacteria, bifidobacteria and yeasts and influence of low-pH stress exposure on their selenium biotransformation efficiency.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Spectrochimica Acta - Part B Atomic Spectroscopy

ISSN

0584-8547

e-ISSN

1873-3565

Volume of the periodical

199

Issue of the periodical within the volume

January 2023

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

106592

UT code for WoS article

000922665300011

EID of the result in the Scopus database

2-s2.0-85143352453