Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F04%3A%230000005" target="_blank" >RIV/26784246:_____/04:#0000005 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.
Original language description
We have used two highly abundant retrotransposons, representative one of the major group of long terminal repeats (LTR) - containing retrotransposons of Ty3/gypsy-like class, Ogre and Cyclop, for development of molecular markers for pea cultivars genotyping. The aim of this study was to access retrotransposon based markers to evaluate the genetic diversity of pea (Pisum sativum L.). To test the utility and reliability of the method we have chosen 20 commercially used pea cultivars with well known pedegree. Due to the sequence knowledge, IRAP markers are better scorable and result in more accurate genetic distance estimations over the REMAP, ISSR and RAPD techniques. With the use of just two outward-facing primers annealing to LTR Ogre and Cyclop targetsequences 56 and 43 polymorphic bands per cultivar and reaction were produced which clearly distinguished and separated 20 investigated pea cultivars into groups related to known pedegree.
Czech name
Využitelnost vysoce kopiových retrotransposonových elementů Ogre a cyclop pro molekulární genotypizaci kolekce hrachu.
Czech description
Pomocí dvou vybraných retrotransposonů Ogre a Cyclop byla vyvinuta metoda vhodná pro studium genetické struktury genofondových sbírek harchu. Výhoda této IRAP techniky ve srovnání v jinými je vysoká specificita a zisk dostatečného množství informací postačujících k odlišení i blízce příbuzných odrůd hrachu
Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Genetic Variation for Plant Breeding
ISBN
3-900962-56-1
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
326
Publisher name
BOKU University, Tull
Place of publication
Vienna, Rakousko
Event location
Tulln , Rakousko
Event date
Jan 1, 2004
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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