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Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F04%3A%230000005" target="_blank" >RIV/26784246:_____/04:#0000005 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.

  • Original language description

    We have used two highly abundant retrotransposons, representative one of the major group of long terminal repeats (LTR) - containing retrotransposons of Ty3/gypsy-like class, Ogre and Cyclop, for development of molecular markers for pea cultivars genotyping. The aim of this study was to access retrotransposon based markers to evaluate the genetic diversity of pea (Pisum sativum L.). To test the utility and reliability of the method we have chosen 20 commercially used pea cultivars with well known pedegree. Due to the sequence knowledge, IRAP markers are better scorable and result in more accurate genetic distance estimations over the REMAP, ISSR and RAPD techniques. With the use of just two outward-facing primers annealing to LTR Ogre and Cyclop targetsequences 56 and 43 polymorphic bands per cultivar and reaction were produced which clearly distinguished and separated 20 investigated pea cultivars into groups related to known pedegree.

  • Czech name

    Využitelnost vysoce kopiových retrotransposonových elementů Ogre a cyclop pro molekulární genotypizaci kolekce hrachu.

  • Czech description

    Pomocí dvou vybraných retrotransposonů Ogre a Cyclop byla vyvinuta metoda vhodná pro studium genetické struktury genofondových sbírek harchu. Výhoda této IRAP techniky ve srovnání v jinými je vysoká specificita a zisk dostatečného množství informací postačujících k odlišení i blízce příbuzných odrůd hrachu

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Genetic Variation for Plant Breeding

  • ISBN

    3-900962-56-1

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    326

  • Publisher name

    BOKU University, Tull

  • Place of publication

    Vienna, Rakousko

  • Event location

    Tulln , Rakousko

  • Event date

    Jan 1, 2004

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article