Rep-PCR-based typing as a tool for tracking of MRSA infection origin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26788462%3A_____%2F12%3A%230000613" target="_blank" >RIV/26788462:_____/12:#0000613 - isvavai.cz</a>
Alternative codes found
RIV/49608851:_____/12:#0000559
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Rep-PCR-based typing as a tool for tracking of MRSA infection origin
Original language description
A performance of rep-PCR fi ngerprinting method with the (GTG)5 primer was explored in order to evaluate its practical application for confi rmation the source of human staphylococci infection. A laboratory worker daily working with the MRSA and Staphylococcus aureus strains has been tested positively for MRSA nosocomial infection. The collection of nine MRSA strains held in the laboratory has been typed with the rep-PCR method. Analysis of the fi ngerprints with the unweighted pairgroup method using arithmetic averages (UPGMA) clustering method revealed presence of six strain clusters with similarity lower than 85%. The fi ngerprint of MRSA strain infecting the human diff ered signifi cantly from remaining fi ngerprints. This provided clear evidence that MRSA strain infecting the personal did not come from the laboratory strains collection. Our experimental results showed rep-PCR with the (GTG)5 primer as an eff ective tool applicable for diff erentiation of individual S. aureus strai
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Universitatis Agriculturae et Silviculturae Mendeleianae Brunensis
ISSN
1211-8516
e-ISSN
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Volume of the periodical
60
Issue of the periodical within the volume
6
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
251-256
UT code for WoS article
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EID of the result in the Scopus database
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