The identification of rumen ciliates using optimized Cryo-SEM approach

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26788462%3A_____%2F24%3AN0000042" target="_blank" >RIV/26788462:_____/24:N0000042 - isvavai.cz</a>

Alternative codes found

RIV/26788462:_____/24:N0000041

Result on the web

<a href="https://www.agrovyzkum.cz/soubory/bulletin/2024/1/bull_1_2024_25-31_malyugina.pdf" target="_blank" >https://www.agrovyzkum.cz/soubory/bulletin/2024/1/bull_1_2024_25-31_malyugina.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The identification of rumen ciliates using optimized Cryo-SEM approach

Original language description

The rumen is inhabited by a dense and highly diverse microbial community, consisting of bacteria, archaea, protozoa and fungi. Feeds consumed by animals are digested with the help of rumen microorganisms, which play an important role in contributing nutrients to the host animal. The present study aims to determine and investigate protozoal ciliate species in the rumen using modern microscopy and propose a new method of rumen microorganism observation and compare it with the classical microscopic method. For these purposes, fresh ruminal liquor was collected from four cannulated cows housed in individual pens. The animals were fed the high-concentrated basal diet consisting of 65.82 % hay, 48.06 % grass silage, and 16.82 % cereal concentrate enriched with minerals and vitamins. The experimental group consisted of three cows whose diet was enriched with 30 g (3.14 g/kg of DM) of lyophilized microalgae Chlorella Vulgaris. Total daily dry matter intake per feeding was 11.9 kg. Rumen liquor samples were collected three hours after the morning feeding twice a week via a rumen cannula with a probe connected to a vacuum pump. After the collection, aliquot samples were immediately transported to the laboratory for further analysis. In this article, we included results of observing protozoal ciliates and their ultrastructures obtained with the optimized cryo-scanning electron microscopy method.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

<a href="/en/project/SS06020190" target="_blank" >SS06020190: Development of an anti-methanogenic feed supplement to mitigate the environmental impact of livestock farming</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Výzkum v chovu skotu

ISSN

0139-7265

e-ISSN

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Volume of the periodical

244

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

25-31

UT code for WoS article

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EID of the result in the Scopus database

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