Reversible immobilisation of engineered molecules by Ni-NTA chelators.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13430%2F03%3A00002211" target="_blank" >RIV/44555601:13430/03:00002211 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Reversible immobilisation of engineered molecules by Ni-NTA chelators.
Original language description
Biomolecules modified by genetic engineering represent a new and powerful approach for obtaining simpler artificial structures with new or improved properties useful for biosensing purposes. In this paper graphite or gold working electrodes were obtainedby several deposition techniques as well as sputtering, screen-printing and chemical gold plating on copper paths. NH2 groups exposed out the surface were obtained by self assembled monolayers (SAM) of 3-aminopropyltriethoxysilane (3-APTES) on graphite screen printed electrodes or cysteamine on sputtered gold or chemically plated gold on copper. The electrochemical synthesis of the SAM mixed with conducting molecular wires of polianyline (PAN) was studied, obtaining higher deposition yields (increased 50 fold), lower treatment time (<1min with respect to 12h of the chemical treatment), and the ability to address the synthesis on only one electrode in a sensor ?-array. The synthesis of the Ni-NTA chelator followed the common scheme: a)gl
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA522%2F03%2F0659" target="_blank" >GA522/03/0659: Improvement of photosynthetic biosenzors and bioassays for routine herbicide residues detection and non-target organisms risk assessment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Sborník z mezinárodní konference Bioelectrochemistry and Bioenergetics
ISBN
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ISSN
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e-ISSN
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Number of pages
2
Pages from-to
222-223
Publisher name
Tip.G. Capponi srl.
Place of publication
Firenze
Event location
Firenze, Italy
Event date
Jun 19, 2003
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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