Reversible immobilisation of engineered molecules by Ni-NTA Chelators

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13430%2F04%3A00002672" target="_blank" >RIV/44555601:13430/04:00002672 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Reversible immobilisation of engineered molecules by Ni-NTA Chelators
Original language description
In this paper electrochemical synthesis of Ni-NTA chelators on Au or Au-graphite electrodes for subsequent immobilisation of (His)6-tagged proteins (PSII as model molecule) is reported with respect to chemical synthesis, obtaining: i)higher Ni-NTA surface density, ii)lower treatment time (1-12min with respect to 16h normally needed for SAM), iii)ability to address the chelator on only one Au electrode in a sensor m-array.
Czech name
Vratná imobilizace rekombinantních molekul na podkladě Ni-NTA vazeb
Czech description
Článek popisuje novou metodu elektrochemicky lokalizované imobilizace biomolekul na povrch elektrodového pole.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/GA522%2F03%2F0659" target="_blank" >GA522/03/0659: Improvement of photosynthetic biosenzors and bioassays for routine herbicide residues detection and non-target organisms risk assessment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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