Monolayers of natural and recombinant photosystem II on gold electrodes - potentials for use as biosensors for detection of herbicides.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13430%2F03%3A00002212" target="_blank" >RIV/44555601:13430/03:00002212 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Monolayers of natural and recombinant photosystem II on gold electrodes - potentials for use as biosensors for detection of herbicides.
Original language description
The herbicides inhibiting the photosystem II (PSII) still represent a basic tool of weed control. Their overuse brings serious environmental and health risks. Since chromatographic methods and immunoassays are not suitable for prescreening assay, we haverecently designed a biosensor based on isolated PSII from thermophilic cyanobacteria Synechococcus elongatus1. The performance of this set-up is restricted by crosslinking the PSII in a gel matrix of bovine serum albumine (BSA) and glutaraldehyde (GA).Gel entrapment resulted in slow mediator diffusion and decreased sensitivity to herbicides. An oriented monolayer of PSII could bring entirely new properties such as fast mediator transport, increased sensitivity and spatially controlled immobilization.Here we present the results of two methods of monolayer immobilization of PSII and prospects for its use as a biosensor for detection of herbicides: (i) Immobilisation based on the recombinant (His)6-tagged PSII;(ii) Immobilisation based
Czech name
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Czech description
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Project
<a href="/en/project/GA522%2F03%2F0659" target="_blank" >GA522/03/0659: Improvement of photosynthetic biosenzors and bioassays for routine herbicide residues detection and non-target organisms risk assessment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Sborník z mezinárodní konference Biosensors for Food Safety and Environmental Monitoring
ISBN
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ISSN
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e-ISSN
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Number of pages
2
Pages from-to
11-12
Publisher name
LACB, Faculte des Sciences et Techniques Universite Hassan II - Mohammedia
Place of publication
Marrakech
Event location
Marrakech
Event date
Sep 9, 2003
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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