Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siRNA delivery systems

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F11%3A43878440" target="_blank" >RIV/44555601:13440/11:43878440 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271103&_user=640945&_pii=S0168365911001015&_check=y&_origin=&_coverDate=15-Jul-2011&view=c&wchp=dGLzVlV-zSkzS&md5=83f002ff8b371e9ba8bb36169e9d3ff8/1-s2.0-S0168365911001015-main.pdf" target="_blank" >http://www.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271103&_user=640945&_pii=S0168365911001015&_check=y&_origin=&_coverDate=15-Jul-2011&view=c&wchp=dGLzVlV-zSkzS&md5=83f002ff8b371e9ba8bb36169e9d3ff8/1-s2.0-S0168365911001015-main.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jconrel.2011.02.016" target="_blank" >10.1016/j.jconrel.2011.02.016</a>

Result language
angličtina
Original language name
Molecular modeling and in vivo imaging can identify successful flexible triazine dendrimer-based siRNA delivery systems
Original language description
This study aimed to identify suitable siRNA delivery systems based on flexible generation 2-4 triazine dendrimers by correlating physico-chemical and biological in vitro and in vivo properties of the complexes with thermodynamic parameters calculated using molecular modeling. The siRNA binding properties of the dendrimers and PEI 25 kDa were simulated, binding and stability were measured in SYBR Gold assays, and hydrodynamic diameters, zeta potentials, and cytotoxicity were quantified. These parameterswere compared with cellular uptake of the complexes and their ability to mediate RNAi. Radiolabeled complexes were administered intravenously, and pharmacokinetic profiles and biodistribution of these polyplexes were assessed both invasively and non-invasively. All flexible triazine dendrimers formed thermodynamically more stable complexes than PEI. While PEI and the generation 4 dendrimer interacted more superficially with siRNA, generation 2 and 3 virtually coalesced with siRNA, formin
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
<a href="/en/project/OC10053" target="_blank" >OC10053: DENDRIMERS IN BIOMEDICAL APPLICATIONS</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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