Modified Methacrylate Hydrogels Improve Tissue Repair after Spinal Cord Injury
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F18%3A43893779" target="_blank" >RIV/44555601:13440/18:43893779 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/18:00493052 RIV/61389013:_____/18:00493052 RIV/44555601:13450/18:43893779 RIV/00216208:11130/18:10383521
Result on the web
<a href="http://www.mdpi.com/1422-0067/19/9/2481/htm" target="_blank" >http://www.mdpi.com/1422-0067/19/9/2481/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms19092481" target="_blank" >10.3390/ijms19092481</a>
Alternative languages
Result language
angličtina
Original language name
Modified Methacrylate Hydrogels Improve Tissue Repair after Spinal Cord Injury
Original language description
Methacrylate hydrogels have been extensively used as bridging scaffolds in experimental spinal cord injury (SCI) research. As synthetic materials, they can be modified, which leads to improved bridging of the lesion. Fibronectin, a glycoprotein of the extracellular matrix produced by reactive astrocytes after SCI, is known to promote cell adhesion. We implanted 3 methacrylate hydrogels: a scaffold based on hydroxypropylmethacrylamid (HPMA), 2-hydroxyethylmethacrylate (HEMA) and a HEMA hydrogel with an attached fibronectin (HEMA-Fn) in an experimental model of acute SCI in rats. The animals underwent functional evaluation once a week and the spinal cords were histologically assessed 3 months after hydrogel implantation. We found that both the HPMA and the HEMA-Fn hydrogel scaffolds lead to partial sensory improvement compared to control animals and animals treated with plain HEMA scaffold. The HPMA scaffold showed an increased connective tissue infiltration compared to plain HEMA hydrogels. There was a tendency towards connective tissue infiltration and higher blood vessel ingrowth in the HEMA-Fn scaffold. HPMA hydrogels showed a significantly increased axonal ingrowth compared to HEMA-Fn and plain HEMA; while there were some neurofilaments in the peripheral as well as the central region of the HEMA-Fn scaffold, no neurofilaments were found in plain HEMA hydrogels. In conclusion, HPMA hydrogel as well as the HEMA-Fn scaffold showed better bridging qualities compared to the plain HEMA hydrogel, which resulted in very limited partial sensory improvement.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30210 - Clinical neurology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
9
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
"nestrankovano"
UT code for WoS article
000449988100017
EID of the result in the Scopus database
2-s2.0-85052521710