Modified biosensor for cholinesterase inhibitors with Guinea Green B as the colour indicator

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F45808121%3A_____%2F18%3AN0000006" target="_blank" >RIV/45808121:_____/18:N0000006 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Modified biosensor for cholinesterase inhibitors with Guinea Green B as the colour indicator
Original language description
Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable and very simple detection of agents in air, in water and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualised by using Ellman’s reagent as a chromogenic indicator. The modification basically consists of a much more distinct colour response of the biosensor, attained through optimisation of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 seconds, inhibition efficiency 25%).
Czech name
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Czech description
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Project
<a href="/en/project/VI20152018024" target="_blank" >VI20152018024: NEW TECHNOLOGIES FOR PERSONAL DETECTOR OF CHOLINE ESTERASE INHIBITORS</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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