Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46356606%3A_____%2F18%3AN0000002" target="_blank" >RIV/46356606:_____/18:N0000002 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/18:00495972 RIV/68378050:_____/18:00495972
Result on the web
<a href="https://academic.oup.com/ps/article/97/4/1127/4801132" target="_blank" >https://academic.oup.com/ps/article/97/4/1127/4801132</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3382/ps/pex411" target="_blank" >10.3382/ps/pex411</a>
Alternative languages
Result language
angličtina
Original language name
Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay
Original language description
A chicken multiplex cytokine assay (Bio-Plex) to detect four different cytokines (IL- 2, IL-12, IL-10, and interferon gamma) simultaneously in plasma samples was designed. Most standard curves range between 1 to 5 pg/mL and 5,000 pg/mL, except for IFNγ with the range of 50 to 25,000 pg/mL. Such a chicken multiplex assay proved to be fast and reliable, and comparable in sensitivity, accuracy, and reproducibility to conventional enzyme-linked immunosorbent assays. Comparison of the multiplex assay with the ELISA technique using the same clones of detection and capture antibodies resulted in correlation coefficients for all cytokines ranging from 0.95 to 0.99. Lower limit of detection and limit of quantification values were obtained for all tested cytokines by the Bio-Plex assay compared with ELISA. To reduce the risk of cross-reaction with other proteins, the Bio-Plex system was used, combining the principle of sandwich immunoassay with the Luminex bead-based technology. The cytokine standard recoveries for each cytokine varied between 86 and 118% in dynamic concentration ranges. A chicken multiplex cytokine assay (Bio-Plex) provided a more complete picture of differences between the Th1/Th2 cytokine profiles of the immunized via a new system of antigen delivery into chicken antigen-presenting cells and control groups. This multiplexed fluorescent-bead-based detection assay can be used as a quantitative or comparative tool for the study of the chicken ex vivo cellular immune response.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Poultry Science
ISSN
0032-5791
e-ISSN
1525-3171
Volume of the periodical
97
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
1127-1133
UT code for WoS article
000427704900005
EID of the result in the Scopus database
2-s2.0-85051691092