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Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46356606%3A_____%2F18%3AN0000002" target="_blank" >RIV/46356606:_____/18:N0000002 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/18:00495972 RIV/68378050:_____/18:00495972

  • Result on the web

    <a href="https://academic.oup.com/ps/article/97/4/1127/4801132" target="_blank" >https://academic.oup.com/ps/article/97/4/1127/4801132</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3382/ps/pex411" target="_blank" >10.3382/ps/pex411</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Simultaneous detection of chicken cytokines in plasma samples using the Bio-Plex assay

  • Original language description

    A chicken multiplex cytokine assay (Bio-Plex) to detect four different cytokines (IL- 2, IL-12, IL-10, and interferon gamma) simultaneously in plasma samples was designed. Most standard curves range between 1 to 5 pg/mL and 5,000 pg/mL, except for IFNγ with the range of 50 to 25,000 pg/mL. Such a chicken multiplex assay proved to be fast and reliable, and comparable in sensitivity, accuracy, and reproducibility to conventional enzyme-linked immunosorbent assays. Comparison of the multiplex assay with the ELISA technique using the same clones of detection and capture antibodies resulted in correlation coefficients for all cytokines ranging from 0.95 to 0.99. Lower limit of detection and limit of quantification values were obtained for all tested cytokines by the Bio-Plex assay compared with ELISA. To reduce the risk of cross-reaction with other proteins, the Bio-Plex system was used, combining the principle of sandwich immunoassay with the Luminex bead-based technology. The cytokine standard recoveries for each cytokine varied between 86 and 118% in dynamic concentration ranges. A chicken multiplex cytokine assay (Bio-Plex) provided a more complete picture of differences between the Th1/Th2 cytokine profiles of the immunized via a new system of antigen delivery into chicken antigen-presenting cells and control groups. This multiplexed fluorescent-bead-based detection assay can be used as a quantitative or comparative tool for the study of the chicken ex vivo cellular immune response.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40301 - Veterinary science

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Poultry Science

  • ISSN

    0032-5791

  • e-ISSN

    1525-3171

  • Volume of the periodical

    97

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    1127-1133

  • UT code for WoS article

    000427704900005

  • EID of the result in the Scopus database

    2-s2.0-85051691092