Finite element analysis of microfluidic device for Escherichia coli separation from blood-cells for early diagnosis of sepsis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24210%2F25%3A00013740" target="_blank" >RIV/46747885:24210/25:00013740 - isvavai.cz</a>

Alternative codes found

RIV/46747885:24620/25:00013740

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S2590123025026271?pes=vor&utm_source=scopus&getft_integrator=scopus" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2590123025026271?pes=vor&utm_source=scopus&getft_integrator=scopus</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.rineng.2025.106558" target="_blank" >10.1016/j.rineng.2025.106558</a>

Result language

angličtina

Original language name

Finite element analysis of microfluidic device for Escherichia coli separation from blood-cells for early diagnosis of sepsis

Original language description

Blood cell separation and infectious microorganism detection were investigated using COMSOL Multiphysics (R) 5.3a simulation software for finite element analysis (FEA). FEA was used as a preliminary study to optimize physical prototypes and experiments using related numerical techniques. The separation of white blood cells (WBCs) from other particles in the blood sample was achieved through solid-phase microextraction, which relies on size-based separation, whereas the detection of infectious microorganisms was based on their electrical properties. Both these separation and detection methods can be implemented on a microfluidic device with simple processes. The FEA studies demonstrate an efficient microfluidic channel design for optimized flow, enabling blood cell separation through microchannel width manipulation and the detection of infectious microorganisms via applied dielectrophoretic force. The simulation results indicated that a flow rate of 0.05 mL/ min at 3 MHz yields a high recovery efficiency of 85 %, with separation accomplished in below 10 min. Furthermore, fluorescence-based cell counting confirmed a WBC count of 2.0 x 109/L in the designated counting channel, aligning well with the simulation results. The findings proved that early diagnosis of sepsis is effectively possible through rapid WBC analysis and identification of specific infectious microorganisms using a microfluidic device, enabling timely point-of-care treatment without side effects.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

21100 - Other engineering and technologies

Project

<a href="/en/project/EF16_025%2F0007293" target="_blank" >EF16_025/0007293: Modular platform for autonomous chassis of specialized electric vehicles for freight and equipment transportation</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Results in Engineering>

ISSN

2590-1230

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

SEP

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

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UT code for WoS article

001544957000001

EID of the result in the Scopus database

2-s2.0-105012267348