Inorganic Silica Nanofibers in a Role of Bioactive Compound Carrier

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24220%2F15%3A00003403" target="_blank" >RIV/46747885:24220/15:00003403 - isvavai.cz</a>
Alternative codes found
RIV/46747885:24620/15:00003403
Result on the web
<a href="http://www.nanocon.eu/files/proceedings/23/papers/4485.pdf" target="_blank" >http://www.nanocon.eu/files/proceedings/23/papers/4485.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Inorganic Silica Nanofibers in a Role of Bioactive Compound Carrier
Original language description
This study is focused on enzyme immobilization on nanofibers (NF). NF are valued for their ultra-high specific surface areas and have been found potentially useful in many biomedical applications. A spectrum of bioactive molecules, including antibacterial agents, anti-cancer drugs, enzymes, proteins, can be incorporated into/onto nanofibers via different approaches. Enzyme immobilization providing many advantages over use of its soluble form: enhanced enzyme activity, reusability, simplified processing, better storage properties and pH stability. We describe the immobilization of trypsin (EC3.4.21.4) on silica nanofibers. After silanisation incorporating 72 ± 12.6 μg NH2 per mg of sheets, trypsin was bound using combinatory chemistry of EDAC and sulfo-NHS. The amount (215 ± 29.8 μg protein/cm2) and activity (24.8 ± 2.7 I.U./cm2) of immobilized biocatalyst as well as storage and operational stability were measured. The proteolytic activity of immobilized trypsin determined immediately after immobilization and during one week was high and stable. Prepared bio-functional nanofiber material can find the application in wound healing process (enzyme debridement).
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
NANOCON 2015 - 7th International Conference on Nanomaterials - Research and Application
ISBN
978-80-87294-63-5
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
413-418
Publisher name
TANGER Ltd.
Place of publication
Ostrava
Event location
Brno
Event date
Jan 1, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000374708800073

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