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Human Osteoblast-like Saos-2 Cells on Submicron-scale Fibers Coated with Nanocrystalline Diamond Films

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24410%2F21%3A00008427" target="_blank" >RIV/46747885:24410/21:00008427 - isvavai.cz</a>

  • Alternative codes found

    RIV/46747885:24620/21:00008427 RIV/68378271:_____/21:00541653 RIV/67985823:_____/21:00541653 RIV/68407700:21110/21:00345278 RIV/68407700:21460/21:00345278

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0928493120337115?via=ihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0928493120337115?via=ihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.msec.2020.111792" target="_blank" >10.1016/j.msec.2020.111792</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Human Osteoblast-like Saos-2 Cells on Submicron-scale Fibers Coated with Nanocrystalline Diamond Films

  • Original language description

    Article in IF journal with the following wording of the abstract.... A unique composite nanodiamond-based porous material with a hierarchically-organized submicron-nano-structure was constructed for potential bone tissue engineering. This material consisted of submicron fibers prepared by electrospinning of silicon oxide (SiOx), which were oxygen-terminated (O-SiOx) and were hermetically coated with nanocrystalline diamond (NCD) films. The NCD films were then terminated with hydrogen (H-NCD) or oxygen (O-NCD). The materials were tested as substrates for the adhesion, growth and osteogenic differentiation of human osteoblast-like Saos-2 cells. The number and the spreading area of the initially adhered cells, their growth rate during 7 days after seeding and the activity of alkaline phosphatase (ALP) were significantly higher on the NCD-coated samples than on the uncoated O-SiOx samples. In addition, the concentration of type I collagen was significantly higher in the cells on the O-NCD-coated samples than on the bare O-SiOx samples. The observed differences could be attributed to the tunable wettability of NCD and to the more appropriate surface morphology of the NCD-coated samples in contrast to the less stable, rapidly eroding bare SiOx surface. The H-NCD coatings and the O-NCD coatings both promoted similar initial adhesion of Saos-2 cells, but the subsequent cell proliferation activity was higher on the O-NCD-coated samples. The concentration of beta-actin, vinculin, type I collagen and alkaline phosphatase (ALP), the ALP activity, and also the calcium deposition tended to be higher in the cells on the O-NCD-coated samples than on the H-NCD-coated samples, although these differences did not reach statistical significance. The improved cell performance on the O-NCD-coated samples could be attributed to higher wettability of these samples (water drop contact angle less than 10°), while the H-NCD-coated samples were hydrophobic (contact angle >70°). NCD-coated porous SiOx meshes can therefore be considered as appropriate scaffolds for bone tissue engineering, particularly those with an O-terminated NCD coating.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Materials Science and Engineering C

  • ISSN

    0928-4931

  • e-ISSN

  • Volume of the periodical

    121

  • Issue of the periodical within the volume

    February

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    15

  • Pages from-to

  • UT code for WoS article

    000619124600007

  • EID of the result in the Scopus database

    2-s2.0-85098711683