Influence of Fiber Diameter of Polycaprolactone Nanofibrous Materials on Biofilm Formation and Retention of Bacterial Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24510%2F24%3A00012900" target="_blank" >RIV/46747885:24510/24:00012900 - isvavai.cz</a>

Alternative codes found

RIV/46747885:24620/24:00012900 RIV/60461373:22310/24:43929054 RIV/60461373:22330/24:43929054

Result on the web

<a href="https://pubs.acs.org/doi/10.1021/acsami.4c03642" target="_blank" >https://pubs.acs.org/doi/10.1021/acsami.4c03642</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsami.4c03642" target="_blank" >10.1021/acsami.4c03642</a>

Result language

angličtina

Original language name

Influence of Fiber Diameter of Polycaprolactone Nanofibrous Materials on Biofilm Formation and Retention of Bacterial Cells

Original language description

To develop microbiologically safe nanofibrous materials, it is crucial to understand their interactions with microbial cells. Current research indicates that the morphology of nanofibers, particularly the diameter of the fibers, may play a significant role in biofilm formation and retention. However, it has not yet been determined how the fiber diameter of poly-ε-caprolactone (PCL), one of the most widely used biopolymers, affects these microbial interactions. In this study, two nanofibrous materials electrospun from PCL (PCL45 and PCL80) with different fiber diameter and characteristic distance δ between fibers were compared in terms of their ability to support or inhibit bacterial biofilm formation and retain bacterial cells. Strains of Escherichia coli (ATCC 25922 and ATCC 8739) and Staphylococcus aureus (ATCC 25923 and ATCC 6538) were used as model bacteria. Biofilm formation rate and retention varied significantly between the E. coli and S. aureus strains (p < 0.05) for the tested nanomaterials. In general, PCL showed a lower tendency to be colonized by the tested bacteria compared to the control material (polystyrene). Fiber diameter did not influence the biofilm formation rate of S. aureus strains and E. coli 25922 (p > 0.05), but it did significantly impact the biofilm formation rate of E. coli 8739 and biofilm morphology formed by all of the tested bacterial strains. In PCL45, thick uniform biofilm layers were formed preferably on the surface, while in PCL80 smaller clusters formed preferably inside the structure. Further, fiber diameter significantly influenced the retention of bacterial cells of all the tested strains (p < 0.001). PCL45, with thin fibers (average fiber diameter of 376 nm), retained up to 7 log (CFU mL–1) of staphylococcal cells (100% retention). The overall results indicate PCL45’s potential for further research and highlight the nanofibers’ morphology influence on bacterial interactions and differences in bacterial strains’ behavior in the presence of nanomaterials.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21001 - Nano-materials (production and properties)

Project

<a href="/en/project/GA23-05154S" target="_blank" >GA23-05154S: Investigation of prokaryotic and eukaryotic cell interactions with nanofibers differing in morphology and structure</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ACS Applied Materials & Interfaces

ISSN

1944-8244

e-ISSN

—

Volume of the periodical

16

Issue of the periodical within the volume

20

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

25813-25824

UT code for WoS article

001225155800001

EID of the result in the Scopus database

2-s2.0-85192825154