Application of MTT assay for probing metabolic activity in bacterial biofilm-forming cells on nanofibrous materials

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24510%2F24%3A00012901" target="_blank" >RIV/46747885:24510/24:00012901 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/24:43929782

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0167701224001222?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0167701224001222?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mimet.2024.107010" target="_blank" >10.1016/j.mimet.2024.107010</a>

Result language

angličtina

Original language name

Application of MTT assay for probing metabolic activity in bacterial biofilm-forming cells on nanofibrous materials

Original language description

The quantification of cellular metabolic activity via MTT assay has become a widespread practice in eukaryotic cell studies and is progressively extending to bacterial cell investigations. This study pioneers the application of MTT assay to evaluate the metabolic activity of biofilm-forming cells within bacterial biofilms on nanofibrous materials. The biofilm formation of Staphylococcus aureus and Escherichia coli on nanomaterials electrospun from polycaprolactone (PCL), polylactic acid (PLA), and polyamide (PA) was examined. Various parameters of the MTT assay were systematically investigated, including (i) the dissolution time of the formed formazan, (ii) the addition of glucose, and (iii) the optimal wavelength for spectrophotometric determination. Based on interim findings, a refined protocol suitable for application to nanofibrous materials was devised. We recommend 2 h of the dissolution, the application of glucose, and spectrophotometric measurement at 595 nm to obtain reliable data. Comparative analysis with the reference CFU counting protocol revealed similar trends for both tested bacteria and all tested nanomaterials. The proposed MTT protocol emerges as a suitable method for assessing the metabolic activity of bacterial biofilms on PCL, PLA, and PA nanofibrous materials.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project

<a href="/en/project/GA23-05154S" target="_blank" >GA23-05154S: Investigation of prokaryotic and eukaryotic cell interactions with nanofibers differing in morphology and structure</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microbiological Methods

ISSN

01677012

e-ISSN

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Volume of the periodical

224

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

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UT code for WoS article

001289624000001

EID of the result in the Scopus database

2-s2.0-85200228297