Graphene 2D platform is safe and cytocompatibile for HaCaT cells growing under static and dynamic conditions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24530%2F22%3A00010422" target="_blank" >RIV/46747885:24530/22:00010422 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/22:10448697

Result on the web

<a href="https://pubmed.ncbi.nlm.nih.gov/36170236/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/36170236/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/17435390.2022.2127128" target="_blank" >10.1080/17435390.2022.2127128</a>

Result language

angličtina

Original language name

Graphene 2D platform is safe and cytocompatibile for HaCaT cells growing under static and dynamic conditions

Original language description

The study concerns the influence of graphene monolayer, as a 2 D platform, on cell viability, cytoskeleton, adhesions sites andmorphology of mitochondria of keratinocytes (HaCaT) under static conditions. Based on quantitative and immunofluorescent analysis, it could be stated that graphene substrate does not cause any damage to membrane or disruption of other monitored parameters. Spindle poles and cytokinesis bridges indicating proliferation of cells on this graphene substrate were detected. Moreover, the keratinocyte migration rate on the graphene substrate was comparable to control glass substrate when the created wound was completely closed after 38 hours. HaCaT morphology and viability were also assessed under dynamic conditions (lab on a chip - micro scale). For this purpose, microfluidic graphene system was designed and constructed. No differences as well as no anomalies were observed during cultivation of these cells on the graphene or glass substrates in relation to cultivation conditions: static (macro scale) and dynamic (micro scale). Only natural percentage of dead cells was determined using different methods, which proved that the graphene as the 2 D platform is cytocompatible with keratinocytes. The obtained results encourage the use of the designed lab on a chip system in toxicity testing of graphene also on other cells and further research on the use of graphene monolayers to produce bio-bandages for skin wounds in animal tests.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30108 - Toxicology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

NANOTOXICOLOGY

ISSN

1743-5390

e-ISSN

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Volume of the periodical

16

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

19

Pages from-to

610-628

UT code for WoS article

000861274500001

EID of the result in the Scopus database

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