Graphene monolayer as an appropriate substrate for mesenchymal stem cells support in regenerative medicine
Result description
Synergistic effect of mesenchymal stem cells and graphene monolayer is an undiscovered topic and crucial in the context of creating a graphene skin dressing. Moreover, capturing early changes in the cytoskeleton may prove to be a valuable indicator of cell condition after direct contact with the biomaterial. In this context, here, we investigated the response of human bone marrow mesenchymal stem cells (hBM-MSCs) to graphene monolayer as a scaffold, based on cytoskeleton architecture. Three main components of the cytoskeleton — microfilaments, intermediate filaments and microtubules were characterized as well as analyzed its entire cell morphology (focal adhesions). Based on microscopic analysis and cell area measurements, it can be stated that graphene did not cause a damage or disturbance of any cytoskeleton members and linker proteins (plectin, vinculin) and gap junction protein (connexin 43). hBM-MSCs growing on the graphene monolayer were more spread than cells growing on the glass but without any other significant difference. This suggested that graphene substrate as a monolayer could be a structural reinforcement for MSCs cultivation without affecting their cytoskeleton, which determines cell integrity, connections and migration, all important cell properties in the process of skin wound healing.
Keywords
Connexin 43Focal contactsIntermediate filamentsMicrofilamentsMicrotubulesPlectin
The result's identifiers
Result code in IS VaVaI
Alternative codes found
RIV/00216208:11110/23:10465974
Result on the web
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
Graphene monolayer as an appropriate substrate for mesenchymal stem cells support in regenerative medicine
Original language description
Synergistic effect of mesenchymal stem cells and graphene monolayer is an undiscovered topic and crucial in the context of creating a graphene skin dressing. Moreover, capturing early changes in the cytoskeleton may prove to be a valuable indicator of cell condition after direct contact with the biomaterial. In this context, here, we investigated the response of human bone marrow mesenchymal stem cells (hBM-MSCs) to graphene monolayer as a scaffold, based on cytoskeleton architecture. Three main components of the cytoskeleton — microfilaments, intermediate filaments and microtubules were characterized as well as analyzed its entire cell morphology (focal adhesions). Based on microscopic analysis and cell area measurements, it can be stated that graphene did not cause a damage or disturbance of any cytoskeleton members and linker proteins (plectin, vinculin) and gap junction protein (connexin 43). hBM-MSCs growing on the graphene monolayer were more spread than cells growing on the glass but without any other significant difference. This suggested that graphene substrate as a monolayer could be a structural reinforcement for MSCs cultivation without affecting their cytoskeleton, which determines cell integrity, connections and migration, all important cell properties in the process of skin wound healing.
Czech name
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Czech description
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Classification
Type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Indian Journal of Experimental Biology
ISSN
0019-5189
e-ISSN
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Volume of the periodical
61
Issue of the periodical within the volume
4
Country of publishing house
IN - INDIA
Number of pages
9
Pages from-to
235-243
UT code for WoS article
000996067000001
EID of the result in the Scopus database
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Basic information
Result type
Jimp - Article in a specialist periodical, which is included in the Web of Science database
OECD FORD
Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Year of implementation
2023