Comparison of the Influence of Nanoparticles on Escherichia Coli and Pseudomonas Aeruginosa Bacterial Populations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F15%3A00000073" target="_blank" >RIV/46747885:24620/15:00000073 - isvavai.cz</a>

Alternative codes found

RIV/46747885:24220/15:#0003354

Result on the web

<a href="http://www.nanocon.eu/files/proceedings/20/reports/3417.pdf" target="_blank" >http://www.nanocon.eu/files/proceedings/20/reports/3417.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of the Influence of Nanoparticles on Escherichia Coli and Pseudomonas Aeruginosa Bacterial Populations

Original language description

As use of nanoparticles continues to increase in various fields of human activity, it becomes increasingly important to understand all interactions that occur between nanoparticles and cells. In this experiment, we explore the influence of several types of nanoparticle on populations of facultative anaerobic bacteria (Escherichia coli) and aerobic bacteria (Pseudomonas aeruginosa). Nanoparticle toxicity (nanoparticle concentration 1 g/l) was evaluated through respirometry and microscopic fluorescence analysis, which allows for observation and comparison of both living and dead cells in a sample. These two methods were supplemented by determination of colony forming units. Basal salt medium (BSM) with differing concentration of glucose and a soya broth medium were used for both bacterial populations as a culture medium. Nanodiamond proved to be the least toxic of the nanoparticles tested (though these contained impurities that may have affected the results), while nanoparticles of praseodymium had the most toxic effect on bacterial populations. A scanning electron microscope (SEM) was used to assess both the appearance and the approximate sizes of the nanoparticles and bacteria. The bacteria clearly differed in size, with E. coli averaging 3-4 µm and P. aeruginosa averaging 1.5 µm. The various nanoparticles ranged between 50 and 200 nm. SEM confirmed colonization of nanoparticle aggregate surfaces by bacteria in almost all samples

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NanoCon 2014, 6th International Conference, Conference Proceedings

ISBN

978-80-87294-53-6

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

707-711

Publisher name

TANGER Ltd

Place of publication

Ostrava

Event location

Brno

Event date

Jan 1, 2014

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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