EFFECT OF ELECTRIC CURRENT ON ORGANOHALIDE RESPIRING BACTERIA FROM CONTAMINATED LOCALITY TREATED BY nZVI
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F18%3A00005689" target="_blank" >RIV/46747885:24620/18:00005689 - isvavai.cz</a>
Alternative codes found
RIV/27283933:_____/18:00005689
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
EFFECT OF ELECTRIC CURRENT ON ORGANOHALIDE RESPIRING BACTERIA FROM CONTAMINATED LOCALITY TREATED BY nZVI
Original language description
Chlorinated ethenes (CEs) are the second most common contaminant worldwide. Remediation procedures combining treatment with nanoscale zero-valent iron (nZVI) and anaerobic microbial degradation supported by electric current belongs to one of the most successful approaches. Here we describe an effect of electric current on indigenous microbial degraders of CE during a field study. Groundwater samples were taken from anode well, four cathode wells and three control wells and isolated DNA was analysed using real-time PCR. Total bacterial biomass was quantified using 16S rDNA gene. Markers of organohalide respiration included Dehalococcoides sp., Desulfitobacterium spp. and vinyl chloride (VC) reductases vcrA and bvcA. Denitrifying bacteria were determined by nirK marker and sulfate reducing bacteria by apsA2. Representative samples were analysed using NGS method targeted to the V4 region of 16S rDNA gene. qPCR analysis of the anode well reveal significant decrease in all tested markers connected to eminent decrease in pH to strongly acidic values. Cathode wells maintained high level of bacterial growth even if there were slight changes in pH. Composition of bacterial communities in the anode and cathode wells strongly differed and changed over time. In the control wells, a long-term stability in composition of bacterial community was observed. To conclude, electric current caused decrease in organohalide bacteria and other markers in the anode well, all other wells showed ongoing organohalide respiration important for CEs degradation.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.
Data specific for result type
Article name in the collection
NANOCON 2017 - Conference Proceedings, 9th International Conference on Nanomaterials - Research and Application
ISBN
9788087294819
ISSN
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e-ISSN
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Number of pages
7
Pages from-to
390-396
Publisher name
TANGER Ltd.
Place of publication
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Event location
Brno
Event date
Jan 1, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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