RNA Extraction from Soil Bacterium Pseudomonas Putida And Green Alga Raphidocelis Subcapitata After Exposure to Nanoscale Zero Valent Iron
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F21%3A00009748" target="_blank" >RIV/46747885:24620/21:00009748 - isvavai.cz</a>
Result on the web
<a href="https://www.confer.cz/nanocon/2020/3764-rna-extraction-from-soil-bacterium-pseudomonas-putida-and-green-alga-raphidocelis-subcapitata-after-exposure-to-nzvi" target="_blank" >https://www.confer.cz/nanocon/2020/3764-rna-extraction-from-soil-bacterium-pseudomonas-putida-and-green-alga-raphidocelis-subcapitata-after-exposure-to-nzvi</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2020.3764" target="_blank" >10.37904/nanocon.2020.3764</a>
Alternative languages
Result language
angličtina
Original language name
RNA Extraction from Soil Bacterium Pseudomonas Putida And Green Alga Raphidocelis Subcapitata After Exposure to Nanoscale Zero Valent Iron
Original language description
Soil bacterium Pseudomonas putida and green alga Raphidocelis subcapitata are often used in ecotoxicology studies, including assessment of nanomaterials. Exposure of these microorganisms to reactive nZVI, however, reduce the quality of extracted RNA for further transcriptomic analysis. In this study, we compare RNA extraction using eight extraction protocols. The extraction using the commercial column kits was often unable to elute both the RNA species in a single elution, whereas phenol-chloroform method using RNAzol recovered both small RNA and large RNA from P. putida. On the other hand, the column based RNA extraction kit from ISOLATE II RNA Mini gave the best yield approximately of 1 µg/µl of RNA and RIN 9.0. RNA extraction from R. subcapitata was more complicated when we used a similar column based RNA extraction system, it required additional and thorough cell lysis steps that included cell lyophilisation and ribolization prior to phenol-chloroform extraction method. Then the yield of an effective RNA concentration ranged from 300 ng/µl to 980 ng/µl, giving the RIN 5.5-7.8. The final protocols solved most of the extraction problems, and allow experiments involving RNA gene expression analysis of microorganisms exposed to reactive nZVI.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LM2018124" target="_blank" >LM2018124: Nanomaterials and Nanotechnologies for Environment Protection and Sustainable Future</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2020)
ISBN
978-80-87294-98-7
ISSN
2694-930X
e-ISSN
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Number of pages
6
Pages from-to
477-482
Publisher name
TANGER LTD
Place of publication
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Event location
Brno, Czech Republic
Event date
Jan 1, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000664505500081