Dissolved Iron Released from Nanoscale Zero-valent Iron (nzvi) Activates the Defense System in Bacterium Pseudomonas Putida, Leading to High Tolerance to Oxidative Stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F22%3A00010136" target="_blank" >RIV/46747885:24620/22:00010136 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0304389422014200" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0304389422014200</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jhazmat.2022.129627" target="_blank" >10.1016/j.jhazmat.2022.129627</a>

Result language

angličtina

Original language name

Dissolved Iron Released from Nanoscale Zero-valent Iron (nzvi) Activates the Defense System in Bacterium Pseudomonas Putida, Leading to High Tolerance to Oxidative Stress

Original language description

Nanoscale zero-valent iron (nZVI) has increasingly been applied to remediate aquifers polluted by organochlorines or heavy metals. As a result, bacteria in the vicinity of remediate action can be stressed by surplus iron released from nZVI. However, the understanding of the iron stress defense pathways during this process is currently incomplete. Therefore, we aimed to elucidate the physiological and transcriptomic response of the bacterium, Pseudomonas putida NCTC 10936, to 100 mg/L of nZVI and 44.5 µg/L of dissolved iron obtained from nZVI suspension. Cell viability was neither affected by nZVI nor dissolved iron, although the dissolved iron caused stress that altered the cell physiology and caused the generation of smaller cells, whereas cells were elongated in the presence of nZVI. Transcriptomic analysis confirmed the observed stronger physiological effect caused by dissolved iron (in total 3839 differentially expressed genes [DEGs]) than by nZVI (945 DEGs). Dissolved iron (but not nZVI) activated genes involved in oxidative stress-related pathways, antioxidant activity, carbohydrate and energy metabolism, but downregulated genes associated with flagellar assembly proteins and two-component systems involved in sensing external stimuli. As a result, bacteria very effectively faced oxidative insults and cell viability was not affected.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20701 - Environmental and geological engineering, geotechnics

Project

<a href="/en/project/EF16_019%2F0000843" target="_blank" >EF16_019/0000843: Hybrid Materials for Hierarchical Structure</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Hazardous Materials

ISSN

0304-3894

e-ISSN

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Volume of the periodical

439

Issue of the periodical within the volume

July

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

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UT code for WoS article

000863521900006

EID of the result in the Scopus database

2-s2.0-85134589990