Tire wear particles enhance horizontal gene transfer of antibiotic resistance genes in aquatic ecosystems
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F24%3A00013034" target="_blank" >RIV/46747885:24620/24:00013034 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0013935124020942#kwrds0010" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013935124020942#kwrds0010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.envres.2024.120187" target="_blank" >10.1016/j.envres.2024.120187</a>
Alternative languages
Result language
angličtina
Original language name
Tire wear particles enhance horizontal gene transfer of antibiotic resistance genes in aquatic ecosystems
Original language description
Microplastics (MPs) have introduced new surfaces for biofilm development and gene exchange among bacteria. We investigated Tire Wear Particles (TWPs) for their involvement in horizontal gene transfer (HGT), particularly in relation to associated metals in the matrices of TWPs. We employed red-fluorescently tagged E. coli strain as a donor with green-fluorescently tagged, broad-host-range plasmid pKJK5, resistant to trimethoprim. As a recipient, we utilized Pseudomonas sp. and a natural lake microbial community. HGT activity on TWPs was determined and compared with that on polystyrene (PS) (with and without metals), and chitosan, which was used as a natural surface. Exposure to TWPs significantly enhanced HGT frequency of antibiotic resistance gene (ARG) from donor to recipient compared to PS and chitosan, and metals of TWPs further promoted HGT. HGT frequency on TWPs with Pseudomonas sp. was found to be 10−3 at 30 °C. in the lake community, it was similarly high already at 25 °C suggesting a higher permissiveness of the natural microbial community towards ARG at lower temperatures. This study sheds light on the potential impact of TWPs in promoting HGT, forming the basis for health risk assessments of TWPs and more generally of MP pollution in various aquatic ecosystems.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10511 - Environmental sciences (social aspects to be 5.7)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Research
ISSN
0013-9351
e-ISSN
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Volume of the periodical
263
Issue of the periodical within the volume
December
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
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UT code for WoS article
001343987400001
EID of the result in the Scopus database
2-s2.0-85207017996