Impact of fluoroquinolone and heavy metal pollution on antibiotic resistance maintenance in aquatic ecosystems

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F25%3A00013588" target="_blank" >RIV/46747885:24620/25:00013588 - isvavai.cz</a>

Result on the web

<a href="https://environmentalmicrobiome.biomedcentral.com/articles/10.1186/s40793-025-00722-5#Abs1" target="_blank" >https://environmentalmicrobiome.biomedcentral.com/articles/10.1186/s40793-025-00722-5#Abs1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s40793-025-00722-5" target="_blank" >10.1186/s40793-025-00722-5</a>

Result language

angličtina

Original language name

Impact of fluoroquinolone and heavy metal pollution on antibiotic resistance maintenance in aquatic ecosystems

Original language description

BackgroundFreshwater pollution with compounds used during anthropogenic activities could be a major driver of antibiotic resistance emergence and dissemination in environmental settings. Fluoroquinolones and heavy metals are two widely used aquatic pollutants that show a high stability in the environment and have well-known effects on antibiotic resistance selection. However, the impact of these compounds on antibiotic resistance maintenance in aquatic ecosystems remains unknown. In this study, we used a microcosm approach to determine the persistence of two fluoroquinolones (ciprofloxacin, ofloxacin) and two heavy metals (copper and zinc) in the Rh & ocirc;ne river over 27 days. In addition, we established links between antibiotic and metal pollution, alone and in combination, and the composition of freshwater bacterial communities, the selection of specific members and the selection and maintenance of antibiotic and metal resistance genes (ARGs and MRGs) using a metagenomics approach.ResultsWhereas ofloxacin was detected at higher levels in freshwater after 27 days, copper had the strongest influence on bacterial communities and antibiotic and metal resistance gene selection. In addition, heavy metal exposure selected for some ARG-harboring bacteria that contained MRGs. Our research shows a heavy metal-driven transient co-selection for fluoroquinolone resistance in an aquatic ecosystem that could be largely explained by the short-term selection of Pseudomonas subpopulations harboring both fluoroquinolone efflux pumps and copper resistance genes.ConclusionThis research highlights the complexity and compound-specificity of dose-response relationships in freshwater ecosystems and provides new insights into the medium-term community structure modifications induced by overall sub-inhibitory levels of antibiotic and heavy metal pollution that may lead to the selection and maintenance of antibiotic resistance in low-impacted ecosystems exposed to multiple pollutants.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

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Continuities

R - Projekt Ramcoveho programu EK

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ENVIRONMENTAL MICROBIOME>

ISSN

2524-6372

e-ISSN

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Volume of the periodical

20

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

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UT code for WoS article

001497872900001

EID of the result in the Scopus database

2-s2.0-105006847293