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Comparison of Methods for Isolation of Bacterial and Fungal DNA from Human Blood

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F47813059%3A19610%2F14%3A%230000402" target="_blank" >RIV/47813059:19610/14:#0000402 - isvavai.cz</a>

  • Result on the web

    <a href="http://link.springer.com/article/10.1007%2Fs00284-013-0451-1" target="_blank" >http://link.springer.com/article/10.1007%2Fs00284-013-0451-1</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s00284-013-0451-1" target="_blank" >10.1007/s00284-013-0451-1</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of Methods for Isolation of Bacterial and Fungal DNA from Human Blood

  • Original language description

    The study aimed at optimization of DNA isolation from blood of representatives of four microbial groups causing sepsis, i.e., Gram negative: Escherichia coli, Gram positive: Staphylococcus aureus, yeast: Candida albicans, and filamentous fungus: Aspergillus fumigatus. Additionally, the five commercial kits for microbial DNA isolation from the blood were tested. The developed procedure of DNA isolation consisted of three consecutive steps, i.e., mechanical disruption, chemical lysis, and thermal lysis. Afterward, DNA was isolated from the previously prepared samples (erythrocyte lysis) with the use of five commercial kits for DNA isolation. They were compared paying heed to detection limit, concentration, DNA purity, and heme concentration in samples. The isolation of DNA without preliminary erythrocyte lysis resulted in far higher heme concentration than when lysis was applied. In the variant with erythrocyte lysis, two of the commercial kits were most effective in purifying the DNA ex

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Current Microbiology

  • ISSN

    0343-8651

  • e-ISSN

  • Volume of the periodical

    68

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    149-155

  • UT code for WoS article

    000329994500003

  • EID of the result in the Scopus database