Comparison of Methods for Isolation of Bacterial and Fungal DNA from Human Blood
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F47813059%3A19610%2F14%3A%230000402" target="_blank" >RIV/47813059:19610/14:#0000402 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007%2Fs00284-013-0451-1" target="_blank" >http://link.springer.com/article/10.1007%2Fs00284-013-0451-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00284-013-0451-1" target="_blank" >10.1007/s00284-013-0451-1</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of Methods for Isolation of Bacterial and Fungal DNA from Human Blood
Original language description
The study aimed at optimization of DNA isolation from blood of representatives of four microbial groups causing sepsis, i.e., Gram negative: Escherichia coli, Gram positive: Staphylococcus aureus, yeast: Candida albicans, and filamentous fungus: Aspergillus fumigatus. Additionally, the five commercial kits for microbial DNA isolation from the blood were tested. The developed procedure of DNA isolation consisted of three consecutive steps, i.e., mechanical disruption, chemical lysis, and thermal lysis. Afterward, DNA was isolated from the previously prepared samples (erythrocyte lysis) with the use of five commercial kits for DNA isolation. They were compared paying heed to detection limit, concentration, DNA purity, and heme concentration in samples. The isolation of DNA without preliminary erythrocyte lysis resulted in far higher heme concentration than when lysis was applied. In the variant with erythrocyte lysis, two of the commercial kits were most effective in purifying the DNA ex
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Microbiology
ISSN
0343-8651
e-ISSN
—
Volume of the periodical
68
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
149-155
UT code for WoS article
000329994500003
EID of the result in the Scopus database
—